BNAI ZION SCIENTISTS DIVISION

JOB ORIENTATION & TERMINOLOGY CLASSES

HPLC TRAINING

Modern HPLC Chromatographic Methods of Chemical Analysis

The aim of the training program is to prepare chemists experienced in the modern HPLC analytical methods. Most care is taken to help students in their job search, taking into account that the main goal is to get and to pass successfully the interview process.

The 6 hours training course includes theoretical and practical parts. Theoretical part is supposed to teach all required basic principles for understanding of the chemistry and physics involved in HPLC. Hands on training includes acquaintance with the equipment. Students are supposed to perform qualitative and quantitative analyses including environmental applications.

HPLC Chromatography

Modes of chromatography. Theoretical aspects of High Performance Liquid Chromatography (HPLC). Analytical and Preparative Liquid Chromatography. Design and parts of the HPLC system. Mobil and stationary phases. Columns, guard columns, detectors, injectors, fraction collectors. HPLC terminology and definitions. Flow rate. Isocratic and gradient, step gradient separations. Degassing. Pressure. Dead volume. Factors affecting band broadening. Peaks asymmetry. Capacity of the column. Effectiveness, Selectivity, Resolution. Theoretical plates. Factors affecting separation. Elution volume. Retention time and Mobil Phase Power. Gaussian curve. Size and porosity of the sorbents. Hydrophilicity and Hidrophobicity of the organic compounds. Pre- and Post-column derivatisation. Sample preparation. Troubleshooting. Quantitative analysis. QA/QC and FDA guidelines.

Dr. Boris Arshava, Ph.D

College of Staten Island,

City University of New York